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ASCP ASCP-MLT Exam Syllabus Topics:

SectionObjectives
Topic 1: Blood Banking / Immunohematology- Blood group systems and antibody identification
  • 1. ABO and Rh systems
    • 2. Crossmatching procedures
      Topic 2: Microbiology- Clinical microbiological techniques
      • 1. Bacteriology and organism identification
        • 2. Culture and sensitivity testing
          Topic 3: Clinical Chemistry- Metabolic and analytical testing
          • 1. Enzymes and electrolytes
            • 2. Endocrine and metabolic panels
              Topic 4: Hematology- Blood cell analysis and disorders
              • 1. Anemias and leukemias overview
                • 2. CBC and differential interpretation
                  Topic 5: Laboratory Operations- Quality and safety in the clinical lab
                  • 1. Quality control and assurance
                    • 2. Laboratory safety and regulations
                      Topic 6: Urinalysis and Body Fluids- Fluid analysis and interpretation
                      • 1. CSF and other body fluids
                        • 2. Urine chemical and microscopic examination

                          ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

                          1. The MCV is indicative of microcytosis with MCV=<80fL. The RDW is within normal limits and indicative of a homogenous cell population.
                          If the MCV was >100 fL, this would be indicative of macrocytosis. An RDW that was outside of normal limits would be indicative of a heterogenous cell population.
                          An 18 year old female has a CBC as part of a routine physical exam. The following results were obtained and the physician determines she is anemic. After reviewing her CBC results shown below, which of the following would be an appropriate description of the anemia?
                          White blood cells (WBC): 5.6 x 10^9/L (RR:4.0-10.0 x 10^9/L)
                          Red blood cells (RBC): 3.7 x 10^12/L (RR: 4.2-5.9 x 10^12/L)
                          Hemoglobin: 9.9 g/dL (RR:12-16 g/dL)
                          Hematocrit: 28% (RR: 37-48%)
                          MCV: 75 fL (80-100 fL)
                          RDW-CV: 14% (RR: 11.0-15.0%)

                          A) Macrocytic, heterogenous
                          B) Microcytic, heterogenous
                          C) Macrocytic, homogenous
                          D) Microcytic, homogenous


                          2. Which of the following cardiac biomarkers rises within 30 minutes - 4 hours after chest pain, peaks in 2 - 12 hours, and is usually normal within 24 - 36 hours.

                          A) Myoglobin
                          B) Cardiac troponins
                          C) CK-MB


                          3. Flow cytometry employs a combination of fluorescent antibody tagging of cells and analysis with laser light scatter.
                          What principle(s) of flow cytometry are employed when performing immunophenotyping:

                          A) Defraction gradients
                          B) Impedance
                          C) Fluorescent antibody tagging and light scatter
                          D) Defraction gradients and impedance


                          4. Name the comprehensive written safety plan detailing the proper use and storage of hazardous chemicals in the workplace.

                          A) chemical hygiene plan
                          B) SOP
                          C) MSDS
                          D) OSHA


                          5. Beta-thalassemia major, also known as Cooley's anemia, has inherited two genes for beta thalassemia without a normal beta-chain gene. This disease is assoicated with a marked deficiency in beta chain production and in the production of normal Hb A. These patients exhibit increased amounts of iron due to the mutliple transfusions that keep them alive. There is also a striking increase in hemoglobin F and an elevation in hemoglobin A2.
                          Hematology
                          A 5-year-old African American child with hepato-splenomegaly and skeletal abnormalities has the following lab results:
                          WBC = 4,800/cu.mm
                          20 NRBC/100 WBC
                          RBC = 2.70 X 106
                          HGB = 6.2 g/dL
                          Many target cells
                          Marked hypochromasia, anisocytosis & poikilocytosis
                          Serum Iron = 200 µg/dL (elevated)
                          Sickle Solubility = negative
                          Hemoglobin F = elevated
                          What is the PROBABLE cause of these findings?

                          A) Sickle cell anemia
                          B) Beta-thalassemia major
                          C) Hemoglobin C disease
                          D) Aplastic anemia


                          Solutions:

                          Question # 1
                          Answer: D
                          Question # 2
                          Answer: A
                          Question # 3
                          Answer: D
                          Question # 4
                          Answer: A
                          Question # 5
                          Answer: B

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